Restriction Enzymes
New England Biolabs, Inc. CviQI – 10000 units
A restriction endonuclease that recognizes the sequence G^TA_C.
New England Biolabs, Inc. Terminal Transferase – 500 units
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT. The 58.3 kDa enzyme does not have 5' or 3' exonuclease activity. The addition of Co2+ in the reacton makes tailing more efficient.
New England Biolabs, Inc. EcoP15I – 500 units
A restriction endonuclease that recognizes the sequence CAGCAGNNNNNNNNNNNNNNNNNNNNNNNNN^NN_.
New England Biolabs, Inc. FatI – 250 units
A restriction endonuclease that recognizes the sequence ^CATG_.
New England Biolabs, Inc. NmeAIII – 250 units
A restriction endonuclease that recognizes the sequence GCCGAGNNNNNNNNNNNNNNNNNNN_NN^.
New England Biolabs, Inc. PspXI – 1000 units
A restriction endonuclease that recognizes the sequence VC^TCGA_GB.
New England Biolabs, Inc. EnGen Spy dCas9 (SNAP-tag) – 400 pmol
EnGen Spy dCas9 is an inactive mutant of Cas9 nuclease that retains programmable DNA binding activity. EnGen Spy dCas9 contains two point mutations, D10A and H840A, which inactivate its RuvC and HNH nuclease domains, respectively. The N-terminal SNAP-tag allows for covalent attachment of fluorophores, biotin, and a number of other conjugates useful for visualization and target enrichment. Like with Cas9 nuclease, DNA binding by EnGen Spy dCas9 requires a guide RNA complementary to a target sequence with a protospacer adjacent motif (PAM), NGG, downstream of the target sequence. EnGen Spy dCas9 contains a SV40 T antigen nuclear localization sequence (NLS) at the C- terminus of the protein.
Supply Solutions Millipore Sigma - Roche Transcriptor Reverse Transcriptase, 1 PK
Millipore Sigma - Roche Transcriptor Reverse Transcriptase, 1 PK
New England Biolabs, Inc. Esp3I – 300 units
A Type IIS restriction endonuclease that recognizes the sequence CGTCTCN^NNNN_....
New England Biolabs, Inc. MluI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence A^CGCGT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BtsI-v2 – 500 units
BtsI-v2 is an engineered and improved version of BtsI that recognizes the sequence GCAGTGNN^.
New England Biolabs, Inc. BssSI-v2 – 200 units
BssSI-v2 is an engineered and improved version of BssSI that recognizes the sequence VC^TCGAGB. BssSI-v2 is an engineered and improved version of BssSI. (NEB uses the designation "v2" or "HF" in the name to indicate engineered enzymes.)
New England Biolabs, Inc. BtsI-v2 – 2500 units
BtsI-v2 is an engineered and improved version of BtsI that recognizes the sequence GCAGTGNN^.
New England Biolabs, Inc. PsiI-v2 – 400 units
A restriction endonuclease that recognizes the sequence TTA_^TAA